Genetic relationship and species identification of Dioscorea polystachya Turcz. in Yams determined by ISSR, ISAP, SRAP and SCAR markers

Dioscorea polystachya Turcz. is the authentic herb Yam in Chinese traditional medicine. In medicinal marketplace, some related species are mislabeled as “Yams”, such D. alata L., exalata C.T. Ting & M.C. Chang, persimili Prain et Burkill, fordii Burkill and japonica Thunb. To determine genetic relationships among these develop a rapid accurate method for identification, Inter Simple Sequence Repeat (ISSR), Intron Amplified Polymorphism (ISAP), Sequence-Related (SRAP) Characterized Region (SCAR) markers were used this study. By combining ISSR, ISAP SRAP markers, high degree of variation was detected. Moreover, relatively gene flow (Nm = 0.3293) observed. This may result from pool exchanges wild materials. The phylogenetic tree similarity analysis showed that had closest relationship with polystachya. Considering lowest inter-species observed highest divergence within japonica, we suggest taxonomy one-species complex, polystachya–D. breeding conservation purposes. data study indicated persimilis independent species, not cultigens. Furthermore, SCAR marker developed could be to discriminate other species. Overall, results useful guidelines programs actions.